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In this study, reduced graphene oxide (rGO) was synthesized from bamboo-derived graphite using Iron (III) nitrate as a catalyst. Bamboo (Gigantochloa albociliata) sourced from Perlis, Malaysia, was selected as a renewable and sustainable biomass precursor. Pyrolysis was carried out under a nitrogen atmosphere at 800°C with a heating rate of 10°C/min and a soaking time of 3 hours. The resulting bamboo charcoal was activated with varying catalyst concentrations (0%, 10%, 20%, and 30%) and subsequently exfoliated and reduced to rGO. Structural and morphological characterizations were conducted using X-ray diffraction (XRD), scanning electron microscopy (SEM), and Raman spectroscopy. The results indicate that a 20% concentration of Iron (III) nitrate yields optimal properties, enhancing graphitization, surface roughness, and porosity while maintaining structural integrity. Additionally, the study demonstrates the potential of bamboo charcoal-derived rGO for applications in water purification, energy storage, electromagnetic wave absorption, and environmental remediation. This work supports the growing demand for low-cost, eco-friendly synthetic graphite, highlighting bamboo's viability as a renewable alternative to non-renewable carbon sources for advanced nanomaterial production.