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ABSTRACT
The conventional detection method such as tissue culture and PCR based analyses are expensive, time-consuming that takes up usually to two weeks, and are labor-intensive. In comparison to conventional methods, the use of microfluidics integrated biosensing system can allow for a rapid identification within possibility of 1-2 hours for confirmation, inexpensive, at a very low amount of concentration. This study outlines an intelligible and unelaborated design for DNA extraction on microfluidics chip for label-free detection Ganoderma boninense, the pathogenic fungus that has been identified to be the major pathogen for palm oil plantation in several countries, especially in Malaysia. cetyltrimethylammonium bromide (CTAB) and AP1 methods of DNA extraction containing different chemical compositions were applied for yield comparison. Results were analyzed using UV-Vis spectroscopy, at different time of sample analysis. DNA profile showed the best using AP1 method with the highest DNA concentration detected on all samples. In comparison, CTAB method showed a smoother spectrum on all samples indicating that the samples most likely contain lesser contaminants compared to AP1 method. Both methods showed peaks at with peaks detected at 275–290 nm, where both dsDNA and ssDNA can be found, indicating successful DNA extraction on microfluidics chip. The electrical measurement carried out on functionalized IDE device also show validated this study. Subsequent optimization of DNA extraction can be further devised for specific applicability of lab-on-a-chip devices.
Keywords: DNA extraction, PDMS, Microfluidics, Ganoderma boninense, Lab-on-a-chip
